Synthesis and a Study of the Antimicrobial Activity of Sulphamic Pectin Derivatives
O. R. AKHMEDOV1, SH. A. SHOMURATOV1, A. S. TURAEV1, and A. VAILI2,3
1Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan
2Xinjiang Technical Institute of Physics and Chemistry, Xinjiang, China
3University of Chinese Academy of Sciences, Beijing, China
Keywords: polysaccharides, pectin, sulphamic acid, periodate oxidation, antimicrobial activity
Pages: 139-143
Abstract
The preparation of new derivatives of polysaccharides showing the biological activity, precisely, antimicrobial properties remains quite a priority task not only in chemistry of high- molecular compounds but also in pharmaceutics and medicine, since some antimicrobial compounds obtained on the basis of natural polymers are superior to synthetic low-molecular weight antimicrobial compounds on some criteria. New water soluble pectin derivatives containing sulphamic groups in their structure were obtained in this work by the interaction of sulphamic acid with polysaccharide aldehyde groups. The structure and composition of the resulting compounds were studied by IR spectroscopy, elemental (nitrogen, sulphur) and X-ray crystal analyses. Sulphamic pectin derivatives with various contents of sulphamic groups were obtained by changing the concentration of sulphamic acid in relation to dialdehyde pectin derivatives. The optimum ratio of reactive components that is -СНО/NH2SO3H = 1.0 : 2.5 and the reaction time of 45 min were found. Study results of antimicrobial action of antimicrobial action of the synthesized sulphamic pectin derivatives were presented. The biological activity of the resulting compounds was studied by the disk diffusion method in relation to gram positive and gram positive negative bacteria. Direct dependence of the antimicrobial activity of the studied preparations on the quantitative content of sulphamic groups in pectin was found. It was determined that sulphamic pectin derivatives with a substitution degree of 35.0 showed antimicrobial activity in relation to Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus vulgaris, Streptococcus faecalis, Streptococcus pyogenes, Streptococcus faecalis, Pseudomonas aeruginosa with a concentration of 50 μg/mL. Antimicrobial action begins to decrease with the decrease in the number of sulphamic groups. Study results of acute toxicity of sulphamic pectin derivatives were presented. According to the results, they can be referred to class V almost nontoxic substances.
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