ROLE OF THE CONSERVATIVE INTERHELICAL HYDROGEN BOND SER74-TRP158 AT THE CHOLESTEROL BINDING SITE IN THE CONFORMATIONAL STABILITY OF THE ОІ2-ADRENERGIC RECEPTOR: MOLECULAR DYNAMICS SIMULATION
T. V. Bogdan, E. S. Alekseev
Lomonosov Moscow State University, Moscow, Russia
Keywords: ОІ-адренорецептор, межспиральная водородная связь Ser74-Trp158, мутация S74A, молекулярно-динамическое моделирование, ОІ-adrenergic receptor, interhelical hydrogen bond Ser74-Trp158, S74A mutation, molecular dynamics simulation
Abstract
To explore the role of the evolutionary conservative interhelical hydrogen bond (IHB) Ser74-Trp158 in maintaining the conformational stability of the β2-adrenergic receptor, this work employs the molecular dynamics technique to study the conformational behavior of the receptor in the presence and absence of IHB. It is found that the Ser74-Trp158 IHB cleavage can cause further conformational transformations (helix shifts, change in the conformation of some amino acid residues). The S74A mutation in the receptor, which makes the IHB formation impossible, also leads to conformational changes: in the presence of cholesterol the mutated receptor takes a conformation close to the active form.
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