THE CARDIOTONIC PROPERTIES OF HUMAN APOLIPOPROTEIN A-I
A.R. Kolpakov1,2, R.A. Knyazev1, N.V. Trifonova1, L.M. Polyakov1
1Institute of Biochemistry, 630117, Novosibirsk, Timakov str., 2
kolpakov2@yandex.ru
2Novosibirsk State Medical University, 63091, Novosibirsk, Krasny av., 52
Keywords: high density lipoproteins, apolipoprotein A-I, isolated rat heart, endogenous cardiotonics
Abstract
The aim of this study was to determine the action of apoliporotein A-I (apoA-I) - the main protein of plasma high density lipoproteins on the work capacity of isolated rat heart and the influence on its effects of classic cardiotoinc drugs (adrenaline, digoxin) and nonselective β-blocker propranolol. Results. By the regime of the recirculation the physiological concentration of apoA-I (20 µg/ml) has stably elevated the intraventricular pressure but the coronary flow was changed insignificantly. Adrenaline has induced two-stage effect: initially it increased the frequency and the force of heart contractions but the induced left ventricular pressure decreased significantly. In the experiments with the simultaneous common perfusion of adrenaline and apoA-I the increased working capacity of isolated heart was recorded during the whole time of observation (30 min). ApoA-I has eliminated the cardiotonc activity of digoxin, and digoxin has removed cardiotonc effect of apoA-I too, and indicators of cardiac activity turned to control parameters but coronary flow was decreased. ApoA-I didn’t abolish the negative chronotropic effect of propranolol but substantially (more 2 fold) increased the left ventricular pressure.
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