LEVELS OF MARKER LYSOSOMAL HYDROLASES IN MEN OF DIFFERENT AGE WITH ISCHEMIC HEART DISEASE THROUGH LEVELS OF SEX STEROIDS
O.V. Tsygankova1, Z.G. Bondareva1, Yu.I. Ragino2, K.Yu. Nikolaev2, D.Yu. Platonov3, V.N. Maksimov2, M.G. Pustovetova1
1Novosibirsk State Medical University, 630091, Novosibirsk, Krasny av., 52
2FSBSI В«Institute of Internal and Preventive Medicine», 630089, Novosibirsk, Boris Bogatkov str., 175/1
3Tver State Medical Academy, 170100, Tver, Sovetskaya str., 4
Keywords: тестостерон, эстрадиол, мужчины, лизосомальные ферменты, ишемическая болезнь сердца, estradiol, testosterone, women, coronary heart disease, lysosomal enzymes
Abstract
Objective: To evaluate the serum concentrations of the three markers of lysosomal hydrolases (cathepsin D, acid phosphatase (AP) and acid DNAse (aDNAase) in men with coronary heart disease (CHD), depending on the levels of testosterone (T), estradiol (E2) and age, examine their association with anthropometric, insulin-glucose parameters of lipid and non-lipid biomarkers of atherosclerosis. The study included 161 women aged 35-65 years, myocardial infarction (MI) is not less than 30 days before the survey. The median age was 53.1 years (25 % 75 % percentage: 40.1 and 59.4 years). The patients were divided into age groups: 35-55 years and 56-65 years (first and second groups, respectively), as well as in groups by levels of sex hormones: T> and ≤ 12 nmol / l and ≥ E2 and <0.194 nmol / l at twofold determination. The results of a comparative analysis and correlation in males 35-55 years of normal T and E2 levels were associated with higher serum concentrations of lysosomal enzymes than those with androdefitsitom and giperestrogeniey and at an older age, on the contrary, androdefitsit and hyperestrogenia lysosomal enzymes accompanied labilization of lysosomal enzymes. In men, CHD patients first age group according to the multivariate analysis three studied lysosomal enzyme inversely dependent on E2 ( p < 0.001) and a number of pro-atherogenic parameters including total cholesterol (TC), low density lipoprotein (LDL) cholesterol, triglycerides (TG), hip circumference (ON), body mass index (BMI), interleukin (IL) -1β ( p < 0.001-0.01), and in 56-65 years a direct impact on the levels of sex steroids lysosomal hydrolases missing, cathepsin D and EC is directly determined by the lipid, non-lipid markers of atherogenesis (TG, high-sensitivity C-reactive protein (hsCRP), malondialdehyde (MDA-30), waist circumference (WC), the index of HOMA-R, p < 0.001-0.05) and back high-density lipoprotein (HDL) cholesterol ( p < 0.001). Acid DNase in both age groups dependent on the parameters involved in the insulin-glucose homeostasis (BMI, ON, TG, index HOMA-R, p < 0.001-0.05), which may reflect a key role in endonuclease accelerated catabolism activatable for various violations of carbohydrate metabolism, up to programmed cell death. Such dynamics of the three markers of lysosomal enzymes demonstrates the different contributions to the development of lysosomal cytotoxicity of CHD in men in the age groups 35-55 and 56-65 years
|
