THE EARLY EVENTS OF ATHEROSCLEROSIS DEVELOPMENT AND THE LEVEL OF FREE CIRCULATING MITOCHONDRIAL DNA IN BLOOD IN THE EXPERIMENTAL DYSLIPIDEMIA
N.P. Sudakov1,2,3, I.V. Klimenkov3,4, T.P. Popkova3, S.B. Nikiforov2, O.A. Goldberg2, S.A. Lepekhova1,2, K.A. Apartsin1,2, A.I. Katyshev5, Yu.M. Konstantinov3,5
1Irkutsk Scientific Center SB RAS, 664033, Irkutsk, Lermontov str., 134
2Irkutsk Scientific Center of Surgery and Traumatology, 664003, Irkutsk, Bortsov Revolyutsii str., 1
3Irkutsk State University, 664003, Irkutsk, Karl Marks str., 1
4Limnological Institute SB RAS, 664033, Irkutsk, Ulan-Batorskaya str., 3
5Siberian Institute of Plant Physiology and Biochemistry, 664033, Irkutsk, Lermontov str., 132
Keywords: дислипопротеидемия, атеросклероз, С-реактивный белок, свободно циркулирующая митохондриальная ДНК, dyslipidemia, atherosclerosis, C-reactive protein, free circulating mitochondrial DNA
Abstract
The aim of research: the studying of blood lipid spectrum, C-reactive protein concentration, and free circulating mitochondrial DNA changes in the course of aorta atherosclerosis development in early steps of high-cholesterol diet in experiment. Material and methods. Dyslipidemia was induced by high-cholesterol diet in rabbits “Chinchilla”. The C-reactive protein level and lipid spectrum was analyzed with Beckman synhron 4 multianalyzer (Beckman coulter, USA). The mitochondrial DNA level was registered by real-time PCR. The ultra structure of aorta surface was studied by scanning electron microscopy. Results. It was shown that in the blood of experimental animals is formed the Beckman imbalance of atherogenic and antiatherogenic cholesterol fractions (on 2 days of dyslipidemia) and iselevated the C-reactive protein level (on the 8 days). Concurrently, on the 16 days of study, we registered the leucocyte adhesion on the aorta surface in the areas of connections with arteria, which could be focuses of atherosclerotic plaque development. The level of free circulating mitochondrial DNA showed the tendency to 3-fold elevation in comparing with control. However, this data predetermined the prospects of blood plasma mitochondrial DNA level studying on the more late stages of dyslipidemia. Conclusion. In the whole, the detected complex of biochemical disturbances of blood and ultrastructural changes of aorta surface in the course of early steps of high-cholesterol diet maybe prospective model of early events of atherosclerosis for pre-clinical translational research in the development of new technologies for diagnostics, prophylaxis, and treatment of lipid metabolism disturbances and atherosclerosis.
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