STRUCTURE AND PROPERTIES OF COMPLEXES OF α–CHYMOTRYPSIN WITH HYDROXYL–CONTAINING GEMINI DICATIONIC SURFACTANTS WITH A SPACER MOIETY OF VARYING LENGTH
Yu. A. Valiullina1, E. A. Ermakova1, D. A. Faizullin1, A. B. Mirgorodskaya2, Yu. F. Zuev1,3
1Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center of the Russian Academy of Sciences, Kazan, Russia
2Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of the Russian Academy of Sciences, Kazan, Russia
3Kazan (Volga) Federal University, Kazan, Russia
Keywords: О±–chymotrypsin, gemini surfactants complexes, structure, catalytic activity
Abstract
The structure and properties of supramolecular complexes of α–chymotrypsin with hydroxyl-containing alkyl ammonium gemini surfactants (GSs) — α,ω–alkanedyl–bis(hydroxyethylmethylcetyl ammonium dibromides), with a polymethylene spacer of varying length have been studied. IR spectroscopy and tryptophan fluorescence data show that the interaction of GSs with α–chymotrypsin leads to changes of different intensity in the structural state of proteins. The most probable complexation mode of enzyme with GSs have been proposed by the molecular docking method. A correlation is found between the activity of α–chymotrypsin and the length of the GS spacer moiety. The enzyme activity correlates with the change in the substrate concentration in the aqueous phase of the surfactant micellar solution.
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