Biodestruction of Hydrocarbons of Highly Viscous Petroleum with Soil Microorganisms
V. S. OVSYANNIKOVA, D. A. FILATOV, L. K. ALTUNINA and L. I. SVAROVSKAYA
Institute of Petroleum Chemistry, Siberian Branch of the Russian Academy of Sciences, Pr. Akademicheskiy 4, Tomsk 634021 (Russia)
Keywords: indigenous soil microflora, enzymatic activity, biodestruction, highly viscous petroleum, hydrocarbon-oxidizing microorganisms, petroleum hydrocarbons, naphthenes, arenes
Abstract
The effect of highly viscous petroleum from the Tzagaan Els deposit (Mongolia) on the oxygenase activity of indigenous soil microflora was studied. It was shown that after the adaptation of microorganisms to hydrocarbons their number increases by two orders of magnitude, while the enzymatic activity increases by a factor of 2–3. Utilization of viscous petroleum during 180 days of experiment is 78 %. Analysis of petroleum after biodegradation showed that it contains a large number of oxygen-containing compounds — intermediate products of oxidation during microbiological assimilation of petroleum hydrocarbons. In the composition of highly viscous petroleum, all hydrocarbons are affected by the biochemical oxidation by soil microorganisms.
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