GENETIC VARIABILITY AND DIFFERENTIATION OF THE KAMCHATKA, SAKHALIN AND KURIL ISLANDS’ LARCHES
I. Yu. Adrianova
Institute of Biology and Soil Science, Russian Academy of Sciences, Far Eastern Branch, Prospekt Stoletiya Vladivostoka, 159, Vladivostok, 690022 Russian Federation
Keywords: larch (Larix), RAPD analysis, genetic variability, genetic differentiation, AMOVA, Kamchatka, Sakhalin, Kuril Islands
Subsection: AN APPLICATION OF MOLECULAR GENETIC METHODS FOR EVALUATION OF GENETIC HETEROGENEITY OF THE MAIN FOREST-FORMING TREE SPECIES’ POPULATIONS
Abstract
The genetic variability and differentiation of the 10 larch populations from Kamchatka, Sakhalin and Iturup Island (Kuril archipelago) were investigated by RAPD method. On the basis of analysis of 319 RAPD loci identified by means of nine random primers it was indicated that the samples from Kamchatka have higher parameters of genetic variability (He = 0.172, P95 = 43.0 %) as compared with the samples from Sakhalin and Iturup (He = 0.142, P95 = 39.5 % and He = 0.143, P95 = 41.1 %, correspondingly). Significant genetic differentiation for the samples from Sakhalin (GST = 0.24) and Kamchatka (GST = 0.25) was revealed. Reliable correlation between genetic and geographic distances between samples was found (r = 0.68, p < 0.005).
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