DEVELOPMENT OF CYTOPLASMATIC SSR-MARKERS FOR POPULATION GENETIC STUDIES OF THE SIBERIAN STONE PINE (PINUS SIBIRICA DU TOUR)
E. A. Shilkina1, N. V. Oreshkova2,3, A. A. Ibe1,3, K. O. Deych1,3, K. V. Krutovsky4,5,6
1Centre for Forest Protection of Krasnoyarsk Territory, Akademgorodok, 50a/2, Krasnoyarsk, 660036 Russian Federation
2V. N. Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch, Akademgorodok, 50/28, Krasnoyarsk, 660036 Russian Federation
3Siberian Federal University, Akademgorodok, 50a/2, Krasnoyarsk, 660036 Russian Federation
4N. I. Vavilov Institute of General Genetics, Russian Academy of Sciences, Akademgorodok, 50a/2, Krasnoyarsk, 660036 Russian Federation
5University of Göttingen, Gubkin str., 3, Moscow, 119333 Russian Federation
6Texas A&M University, Büsgenweg, 2, Göttingen, D-37077 Germany
Keywords: DNA markers, PCR, chloroplast and mitochondrial markers, mitotype, locus, allele, haplotype, Siberian stone pine (Pinus sibirica Du Tour)
Subsection: GENERAL QUESTIONS OF FOREST MOLECULAR GENETICS AND BIOTECHNOLOGY
Abstract
Three chloroplast and one mitochondrial DNA markers were developed and used for genotyping of 60 trees in two populations of the Siberian stone pine (Pinus sibirica Du Tour). Two chloroplast loci were monomorphic in both populations, and one polymorphic with two alleles. Therefore, four chloroplast haplotypes were revealed totally. A mitochondrial DNA marker had two alleles or haplotypes (mitotypes).
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