Cytotoxic and Immunomodulating Properties of Gold Nanoparticles
M. V. KHARKOVA1, A. V. CHEREPANOVA1, E. S. MOROZKIN1, I. A. ZAPOROZHCHENKO1, P. P. LAKTIONOV1, A. A. STRUNOV2, E. V. KISELEVA2, T. G. TOLSTIKOVA3, N. V. SHIKINA4, Z. R. ISMAGILOV4 and V. V. VLASOV1
1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Pr. Akademika Lavrentyeva 8, Novosibirsk 630090 (Russia)
kharkova@niboch.nsc.ru
2Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Pr. Akademika Lavrentyeva 10, Novosibirsk 630090 (Russia)
3Boreskov Institute of Catalysis, Siberian Branch of the Russian Academy of Sciences, Pr. Akademika Lavrentyeva 5, Novosibirsk 630090 (Russia)
4Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of the Russian Academy of Sciences, Pr. Akademika Lavrentyeva 9, Novosibirsk 630090 (Russia)
Keywords: gold nanoparticles, toxicity, immunomodulating properties, primary and transformed cell cultures
Pages: 169–178
Abstract
Results of the investigation of cytotoxic and immunomodulating properties of gold nanoparticles of different sizes (12, 16, 22 and 35 nm) on primary and transformed cells in vitro are presented. Their localization and distribution in cells are studied. It is established that gold nanoparticles are not toxic within concentration range 0.06–6 μg/mL for all the cell cultures used, actively penetrate into the cell and get accumulated inside the vacuoles of different sizes, selectively activate the anti-inflammatory immune response depending on nanoparticle size and cell type.
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