Kinetics of Oxidative Deamination by Monoamine Oxidase Preparations
N. V. GUREEVA
Tyumen State Clinical Academy of Health Ministry of Russia, Ul. Odesskaya 54, Tyumen 625026 (Russia)
E-mail: natalivg@mail.ru
Keywords: serotonin, tryptamine, kinetics, deamination, monoamine oxidase
Pages: 287–291
Abstract
Assessing the activity of monoamine oxidase enzyme was performed for the reactions of serotonin, tryptamine and benzylamine deamination in different groups of animals according the ratio between the maximum deamination rate (wmax) and the Michaelis constant (KM). For the representatives of mammalian species studied the ratio of wmax/KM ranges within 1.0–3.6, for birds this range is wider amounting to 0.4–5.0, for fish this value ranges within 0.3–1.8. It was demonstrated that increasing the concentrations of substrates (up to 10–2 mol/L) or temperature (up to 42 °С) promotes the enzyme activation.
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