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Contemporary Problems of Ecology

2009 year, number 6

Microclonal Propagation and Some Biological Characteristics of Scrophularia umbrosa Dumort. in vitro

G. G. Maystrenko, I. M. Krasnoborov
Keywords: Scrophularia umbrosa, in vitro, growth hormones, microclonаl propagation
Pages: 787-793

Abstract

Peculiarities of sterilization and germinаion of the seeds after different storage periods as explants for introduction into culture in vitro are considered along with the features of morphogenesis and shoot regeneration of S. umbrosa in Murasige and Skuga nutrient medium with the addition of some hormonаl factors. The initial stages of the ontogenesis of fig-wort were studied in vitro. Microclones introduced under the conditions close to those in the natural habitats of the species were under observation.
On the basis of this research, a detailed technology of S. umbrosa micropropagation was elaborated. The reduction of the development cycle and formation of viable seeds in the introduction of microclones developed in vitro was demonstrated.