Cytogenetic characteristics of embryogenic cell lines of different ages obtained via somatic embryogenesis in Larix sibirica Ledeb
M. E. PARK, O. V. GORYACHKINA, I. N. TRETYAKOVA, E. N. MURATOVA
V. N. Sukachev Institute of Forest SB RAS Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russia
Keywords: Larix sibirica, somatic embryogenesis, embryogenic cell lines, number of chromosomes, karyotype, chromosomal and genomic mutations
Abstract
The results of cytogenetic analysis of four embryogenic cell lines of Siberian larch (Larix sibirica Ledeb.) of different ages: young (two four-month and 16-month CLs) and long-proliferating (11-year CL) are presented. Three cell lines are diploid and contain in the karyotype the normal number of chromosomes for this species (2n = 24). A 16-month-old CL capable of forming somatic embryos was aneuploid (2n = 25). This cell line is probably characterized by trisomy on one of the pairs of submetacentric chromosomes. The long-term proliferating CL, from which regenerants and clones were obtained, retains a diploid number of chromosomes during 11 years of cultivation and remains genetically stable. The karyotype of conifers is characterized by high degree of stability and most of the species contain 24 chromosomes (2n = 24). Therefore, the detection of cytogenetically stable cell lines is of great interest for genetic work of this group of plants and creation of highly productive plantations with specified hereditary properties.
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