Home – Home – Jornals – Contemporary Problems of Ecology 2023 number 4

Based on the culture method and high-performance sequencing of the ITS2 rDNA site, the number of colony-forming units (CFU), taxonomic structure and species richness of fungal communities in the bottom ground of Lake Baikal from depths from 9 to 178 m were determined. 215 species of 138 genera of 50 orders of 22 classes of 6 divisions - Ascomycota, Basidiomycota, Mucoromycota, Mortierellomycota, Chytridiomycota, Glomeromycota have been identified. Only DNA barcoding allowed to detect representatives of the Phyla of Chytridiomycota, Glomeromycota and Rozellomycota. The cultural method and high-performance sequencing of the ITS2 rDNA site in the soil revealed 77 and 146 species, respectively, and only 8 species were established by both approaches. Most of the nucleotide sequences have not been identified to the level of families and species. There are representatives of various ecological and trophic groups - saprotrophs, pathogens, symbiotrophs in the mycobiota of the bottom grounds. These are mainly psychrotolerant organisms, among them there are facultative anaerobic species. Changes in the taxonomic structure of fungal communities depending on the depth and species, and able to function in soils, have been established. These are, in particular, Pseudeurotium bakeri, Pseudeurotium hygrophilum, Pseudogymnoascus pannorum, Pseudogymnoascus roseus, Trichoderma hamatum, Trichoderma harzianum, Trichoderma lixii, Trichoderma polysporum, Penicillium glandicola, Penicillium swiecickii, species of the genera Rhizophydium, Fusarium, Daldinia Mortierella, Coniochaeta, Cystobasidium, Mrakia, Rhodothorula, Solicoccozyma and a number of others. A collection of fungal strains isolated from the bottom soils of Lake Baikal has been created.