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Contemporary Problems of Ecology

2022 year, number 5

Influence of abiotic environmental factorson the accumulation ofphenolic metabolitesof Dasiphora fruticosa

E. P. KHRAMOVA
Central Siberian Botanical Garden of SB RAS, Novosibirsk, Russia
Keywords: phenolic compounds, abiotic environmental factors, variability

Abstract

The composition and content of phenolic compounds in leaves of Dasiphora fruticosa plants growing in the Far East, Southern and Eastern Siberia in the range of geographical coordinates 43°13′-62°52ʹ N was studied. The composition of the phenolic complex of D. fruticosa is represented by 16-22 components and remains constant, its variation occurs mainly due to minor components. Seven flavonolglycosides - hyperoside, isoquercitrin, rutin, avicularin, quercitrin, astragalin, kaempferol rutinoside, two aglycones - quercetin and kaempferol, and ellagic acid and its glycoside were determined using HPLC and UV spectrometry. The total content of phenolic compounds in plant leaves ranged from 13.5 to 28.3 mg/g, that of quercetin glycosides from 4.8 mg/g to 17.3 mg/g, and that of kaempferol glycosides from 0.1 to 1.3 mg/g. The total content of ellagic tannins in leaves varied from 6.9 mg/g to 12.9. mg/g. It was found that as D. fruticosa moved from south to north, increased insolation on the background of low-temperature stress, drought, content of all individual components increased, except for avicularin, the content of which, on the contrary, decreased. It was shown that the content of phenolic compounds (in total and in groups) is 53-75 % determined by temperature, of flavonols by 50-91 % by drought and by 50-54 % by solar radiation. Accumulation of ellagic compounds in leaves of D. fruticosa is 60 % determined by temperature, the other factors are of low importance. The factors making a significant contribution to the accumulation of phenolic compounds in D. fruticosa are air temperature, drought and UV radiation