IDENTIFICATION, DIAGNOSTIC AND PHYLOGENETIC ANALYSIS OF VIRUSES VEGETABLE CROPS IN AGROCENOSES AMUR RIVER BASIN (KHABAROVSK TERRITORY)
R.V. Gnutova, V.F. Tolkach, I.B. Nesmelov
Institute of Biology and Soil Science, FEB RAS, 690022, Vladivostok, 100-letia Vladivostok, Pr. 159
girina.vl@mail.ru; ibss@eastnet.febras.ru
Keywords: вирусы растений, мониторинг, идентификация, ПЦР-диагностика, 2b ген, филогенетичес- кий анализ, plant viruses, monitoring, identification, PCR-diagnostic, 2b gene, phylogenetic analysis
Abstract
The results of identification of viruses Bean yellow mosaic virus and Cucumber mosaic virus , identified from vegetable legumes culturales and pumpkin vegetable plants. The possibility of using 2b gene of CMV as a marker for PCR di- agnostics. The study of nucleotide sequences of isolates of CMV and comparative phylogenetic analysеs of isolates from GenBank, showed that the Khabarovsk isolates of CMV consists of subgroup in the IA group I.
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