APPLICATION OF INTER-SIMPLE SEQUENCE REPEAT MARKERS FOR INVESTIGATION OF GENETIC DIVERSITY: A CASE STUDY OF HEDYSARUM THEINUM (FABACEAE)
N.S. Zvyagina, O.V. Dorogina
Central Siberian Вotanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
zviagnat@rambler.ru
Keywords: genetic diversity, Hedysarum theinum, ISSR markers
Abstract
Th e objective of the research described in this paper was to evaluate genetic diversity of endemic species Hedysarum
theinum Krasnob. by inter-simple sequence repeats (ISSR) analysis of genomic DNA. Th ree ISSR markers were used
to analyze the genetic structure of two H. theinum populations from Altai Republic, Russia. Revealed high DNA
polymorphism allows using ISSR technique as a powerful and effi cient approach for research an interpopulation
diversity and genotype fi ngerprinting.
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