DETECTION OF EBOLA VIRUS VP40 PROTEIN USING A SOI BIOSENSOR
V. M. Generalov1, O. V. Naumova2, B. I. Fomin2, S. A. P'yankov1, I. V. Khlistun1, A. S. Safatov1, B. N. Zaitsev1, E. G. Zaitseva2, A. L. Aseev2
1Federal Budgetary Scientific Institution "State Research Center of Virology and Biotechnology "Vector" of the Federal Service for the Oversight of Consumer Protection and Welfare", Novosibirsk Region, Kol'tsovo, Russia
2Rzhanov Institute of Semiconductor Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, Russia
Keywords: биосенсор, кремний-на-изоляторе (КНИ), нанопроволока, индикация, вирус Эбола, белок VP40, biosensor, silicon-on-insulator (SOI), nanowire, indication, Ebola virus, VP40 protein
Abstract
SOI biosensors without surface modification are used to detect the Ebola virus VP40 protein by revealing its immune complexes with specific monoclonal antibodies (MCAs). It is shown that the reaction of specific interaction between VP40 and MCAs gives a more pronounced sensor response than direct adsorption of VP40 or antibodies to their surface. The formation of antigen - antibody complexes leads to the accumulation of a negative charge on the sensor surface. The protein and protein - antibody complexes are detected in real time (∼200-300 s per test sample).
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