DETERMINATION OF THE ORIENTATION OF PHOSPHOLIPIDE MOLECULES IN PLANAR STRUCTURES FROM RAMAN SPECTRA
K. A. Okotrub1, V. A. Zykova1, S. V. Adishchev1, N. V. Surovtsev1,2
1Institute of Automation and Electrometry, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, prosp. akademika Kopytuga, 1
2Novosibirsk State University, 630090, Novosibirsk, ul. Pirogova, 2
Keywords: комбинационное рассеяние света, фосфолипидный бислой, планарная структура, ориентация молекул, Raman scattering, phospholipide bilayer, planar structure, orientation of molecules
Abstract
A method is proposed for determining the orientation of phospholipide molecules in planar structures from spectra of non-polarized Raman scattering. The method is based on the sensitivity of the intensity of lines of Raman scattering on oscillations of CH2 groups to the orientation of phospholipide molecules. The validity of the method is illustrated on a planar specimen of a saturated phospholipide prepared by drying from a solution. It is demonstrated that it is convenient to use the method of principal components to analyze the spatial distribution of molecule orientations in the specimen
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