SYSTEMIC HEMOSTATIC AND PROTHROMBOTIC EFFECTS OF FIBRIN-MONOMER IN EXPERIMENT WITH DOSED LIVER THERAPY
Andrey Pavlovich MOMOT1,2, Vyacheslav Mikhaylovich VDOVIN3,2, Igor Ilyich SHAKHMATOV3,2, Igor Gennadievich TOLSTOKOROV4, Dmitriy Andreevich OREKHOV5, Valentin Olegovich SHEVCHENKO4, Natalya Alexandrovna LYCHEVA3,2, Alexey Vladimirovich KUDINOV3, Galina Gennadievna BELOZERSKAYA6, Valeriy Ivanovich KISELEV3,2
1National Research Center for Hematology of Minzdrav of Russia, Altai Branch
2Research Institute of Physiology and Fundamental Medicine
3Altai State Medical University of Minzdrav of Russia
4Consulting Diagnostic Center В«Dobryi Doktor» Ltd
5Altai Regional Cardiology Health Center
6National Research Center for Hematology of Minzdrav of Russia
Keywords: система гемостаза, фибрин-мономер, травма печени, паренхиматозное кровотечение, гемостатический эффект, кролики, hemostatic system, fibrin monomer, liver injury, parenchymal hemorrhage, hemostatic effect, rabbits
Abstract
Objective of the study is to experimentally evaluate system hemostatic and prothrombotic effects of intravenous fibrin monomer (FM). Materials and methods. Eighty two Chinchilla rabbits weighing 3-4 kg were used in the study. In addition to the placebo, animals were administered an aqueous solution of fibrin monomer (FM) intravenously at doses of 0.1, 0.25, 0.5, 1.0, 2.5 and 5.0 mg/kg. After 1 hour, a standard liver injury was performed and the blood loss (% of the circulating blood volume) resulting from parenchymal hemorrhage was estimated. Hemostatic system examination included platelet number in venous blood and fibrinogen and D-dimer levels in blood plasma. Results and discussion. Blood loss after dosed injury with placebo was 10.0 [4.0; 15.7] % blood volume (median [25th percentile; 75th percentile]). FM preparation doses of 0.25, 2.5 and 5.0 mg/kg resulted in a decrease of blood loss by 6.3 (p < 0.001), 7.8 (p < 0.001) and 2.7 times (p = 0.04) (1.6 [1.0; 3.0], 1.3 [0.6; 1,6] and 3.7 [2.8; 5.3] % blood volume, respectively).High doses of FM preparation (2.5 and 5.0 mg/kg) minimized blood loss due to activation of coagulation and thrombus formation, which was illustrated by a 7.0-8.0-fold increase in D-dimer level (compared to placebo). 0.25 mg/kg of FM preparation did not lead to an increase of D-dimer content, although the blood loss was greatly reduced. Conclusion. The data obtained show the presence of unique hemostatic properties in low-dose FM preparation (0.25 mg/kg), which allows creating system hemostatic agents of a new generation.
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