In vitro Propagation and Conservation of the Rare Species Fritillaria meleagris L. from Floral Explants
D. S. MURASEVA, T. I. NOVIKOVA, A. A. ERST
Central Siberian Botanical Garden, SB RAS, 630090, Novosibirsk, Zolotodolinskaya str., 101
Keywords: Fritillaria meleagris L, in vitro regeneration, floral explants, morpho-histological anаlysis, adventitious shoot formation, biodiversity conservation
Abstract
The peculiarities of in vitro regeneration of the rare species Fritillaria meleagris L. from floral explants was studied for the first time. At the establishment of in vitro culture and during the multiplication stage the most effective was the use of the В5 nutrient medium supplemented with 0.44 mМ BA, 3.22 mМ NAA and 2.28 mМ IAA. During the multiplication stage the regeneration rate reached 80 % and the number of bulblets per explant was 3.9 ± 0.3. It was established that morphogenesis of the de novo formed structures depended on the mineral composition of the medium: the use of mineral-based B5 led to adventitious shoot formation (gemmogenesis), while the use of BDS caused morphogenic callus formation and gemmorhizogenesis on its surface. The stimulating effect of low temperatures (+7 oС) on bulblets’ development and adaptation to ex vitro conditions was noted.
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