The Impact of Ecological and Phytocenotic Conditions on Fraction Composition of Proteins and Sowing Qualities of the Siberian Fir Seeds
S. G. Prokushkin1, V. V. Ermolenko1, Z. V. Erohina2
1V. N. Sukachev Institute of Forest, Russian Academy of Science, Siberian Branch, Akademgorodok, 50/28, Krasnoyarsk, 660036 Russian Federation
2Siberian State Technological University, Prospekt Mira, 82, Krasnoyarsk, 660049 Russian Federation
Keywords: fractional composition of proteins, laboratory germination of seeds, Siberian fir
Abstract
The content and fractional composition of nitrogenous compounds and their role in sowing qualities of the Siberian fir has been identified. In particular: there were significant differences in the content of total protein nitrogen and individual fractions of proteins in seeds of site depending on the fir, the rate of growth of the trees, the location of the seeds in the generative layers of the crown and the vitality of the tree. It was found that the viable seeds of the Siberian fir in middle taiga contains the maximum amount of protein nitrogen, the main fraction of which are sparingly soluble and albumin. Whereas, in the seeds from the southern taiga protein nitrogen content is much less prevalent and sparingly soluble fraction and globulins. A positive correlation between protein content in seeds and their nitrogen laboratory germination component with r = 0.88 and the approximation validity with R2 = 0.77, which is typical for the trees of the Ist, and IVth class of growth by Kraft. Positive relationship has been established between laboratory germination and water-soluble fractions which, according to the researchers, serves as a catalyst. Also a relationship between seed germination and the content of salt–soluble fractions and sparingly soluble fractions, which amounted to 0.93 and 0.76, respectively, has been found. The high content of sparingly soluble protein fraction in the seeds of fir and a very high correlation with its germination is quite natural, as insoluble proteins play a role in improving the energy efficiency of respiratory cells, as well as they are a part of the cellular structures. A correlation between the amount of salt–soluble fraction and germination of seeds has been shown. This correlation is natural, since they are mainly seed storage proteins, for a number of different specific features. The relationship between the content of the alcohol and alkali-soluble protein fractions in the seeds and their germination were insignificant (0.01–0.27), which is also consistent with the data obtained for the Gmelin and Siberian larch seeds.
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