Home – Home – Jornals – The journal "Ateroskleroz" 2010 number 2

Objective: to investigate apolipoprotein AI gene polymorphism and its associations with lipid profile in Caucasian population of West Siberia. The study included 100 persons with total cholesterol >300mg/dl, 100 - with total cholesterol <200mg/dl) and 100 - with total cholesterol 233,6±47,7mg/dl aged 45-64 from sample of Novosibirsk population. The apolipoprotein A1 polymorphism was analyzed by standard method using PCR. The serum lipid levels were determined by standard enzymatic assays. Analysis of promoter polymorphism apolipoprotein A1 (APOAI) gene in three groups showed statistical significance associations between -75G>А of APOAI gene and some key lipid risk factors (total blood cholesterol, low density lipoproteins cholesterol, index of atherogenity) in people of West-Siberian region. The genotype AA has been associated with higher blood lipid levels in population of West Siberia.

Features of the affection of coronary arteries according to the data of selective coronary angiography (SCAG) and multispiral computer tomography (MSCT) of aboriginal and alien population of Yakutia are evaluated. The results of SCAG and MSCT of patients suffering from verified aterosclerosis of coronary arteries (n = 568) were analyzed. It was established that, according to the SCAG data, constriction of the diameter of coronary arteries by 50-75 % and single-vascular affection occur in aboriginal nationalities in the Republic of Sakha more frequently, while the constriction of the diameter of coronary arteries by more than 90 % and affection of three and more coronary arteries occur more rarely than in aliens. It was demonstrated that the medium level of calcium index according to the MSCT data is lower in aboriginal population in comparison with alien population. The frequency of myocardial infarction did not differ in the ethnic groups.

Current study was performed within the HAPIEE project "Determinants of cardiovascular diseases in Eastern Europe". Population-based subsample of 280 subjects  was analyzed. Screenning of TG pathology revealed 16 women with  subclinical hypothyroidism, control group composed of 32 women with euthyriodism was developed by the random numbers method. The received data showed that the total cholesterol level and hs-LDLP were significantly higher comparing with women without TG abnormalities. Characteristics of  vessel wall rigidity (augmentation index in women treated for hypothyroidism or thyrotoxicosis) were similar in participants and control group.

The important biological role reactive oxyradicals is described which are generated in various cells of able-bodied organisms, from unicellular up to highorganized, carrying out, due to a highest chemical reactivity and rather short lifetime, vital function alarm endocellular messengers and intercellular mediators for realization of the expedited and deferred adaptable rearrangement of a metabolism in tissues, including in system of a circulation. At the same time at appreciable accumulation in an organism of such moleculas them mediator function is transformed in damaging, caused by oxidation and destruction of histic lipids, proteins and nucleic acids with development of pathological damages of members and tissues. This concept is compounded with the numerous clinical items of information, that at the patients by an essential hypertension, atherosclerosis, ischemic illness of heart and myocardial infarction histic and the plasma level reactive oxyradicals and products oxidative damage cell's biopolimers is many times higher than sizes observable at the able-bodied people even in the term of extreme situations, and pathological important in this case acts local and - or systemic hyperproduction of superoxidic and hydroxyl radicals, peroxinitrit, oxide of nitrogen, and other awake forms of oxygen.

At the last 30 years the application of the new laboratory species mini-pigs is progressively increasing in medical and biological research of atherosclerosis and other disorders of cardiovascular system. Mini-pigs differ from traditional experimental animals in maximal similarity of their anatomy and physiological functions of cardiovascular, digestive, excretory, and nervous systems with human ones. In the Institute of Cytology and Genetics SB RAS the specialized laboratory mini- and micro-pigs adapted for using under our conditions were created. In 2010 several hundreds of studies on mini-pigs were conducted in Russian Federation and abroad. The present review highlights some experimental works that could be applied for wide use in medicine.