Consumption of Amino Acids by Non-Cultivated Bacterioplankton of an Eutrophic Water Reservoir
O. V. Kolmakova1, M. Y. Trusova2
1 Siberian Federal University
2 Institute of Biophysics of Siberian Branch RAS
mtrusova@ibp.krasn.ru
Keywords: free-living non-cultivated bacterioplankton, amino acids, PCR-DGGE, microecosystems, 16S ribosomal RNA
Pages: 13-21
Abstract
Species (clones) of the water-reservoir bacterioplankton living on lysine and glycine were revealed in the experimental microsystems using unsterile cultivation. The structure of the community was anаlyzed using the separation of amplificated fragments of the 16S рRNA gene of the bacteria under the conditions of denаturating gradient gel electrophoresis. Identification of the dominant species (clones) of bacterioplankton was carried out using molecular cloning and determination of the nucleotide sequence of the region of 16S gene of ribosomal RNA. It was discovered that glycine, one of the prevailing amino acids of an eutrophic water reservoir, and lysine, with its lower content in natural water, served as nutrition substrates for different subdominant species (clones) of bacterioplankton. So, it was established that the species of free-living bacterioplankton are narrow-specialized with respect to the consumption of separate amino acids.
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