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Contemporary Problems of Ecology

2009 year, number 2

Thermodynamic Indices of Active Invertase from Buried Soil Horizons of the Later Pleistocene Age in the North of Yakutia

M. V. Shchelchkova
Keywords: permafrost sediments, later Pleistocene, enzymatic activity of soil, invertase, thermodynamic characteristics, temperature coefficient, activation energy, immobilization
Pages: 195-201

Abstract

New data on the enzymatic activity of the tundra peaty gleyey soil and permafrost sediments on the Later Pleistocene age (11-25 thousand years ago) are analyzed. It is shown that invertase, protease, phosphatase, catalase, peroxidase are well conserved in yedoma and in buried soil horizons; urease is conserved to a smaller extent. On the basis of the investigation of thermodynamic characteristics of active invertase, such as temperature coefficient and activation energy, it was established that this enzyme is present in soil in the free state and in the bound form (immobilized on the surface of organic-mineral colloids). The degree of immobilization of active invertase in the buries soil horizons is high, which is likely to play an important part in the conservation of its molecules at negative temperatures for a long time.