Home – Home – Jornals – Contemporary Problems of Ecology 2004 number 2

The characteristics of callus formation of Pinus sibirica trees under use for work on seed plantation were studied. The results obtained in the investigation allowed us to divide the P. sibirica samples under investigation into two groups. In the I group, callus was formed both on bud and on needles, while in the II group it was formed mainly on buds. Callus obtained from the trees of the I group were characterized by higher growth rate. Necrotic regions on callus in the I group appeared after cultivation for six months as a mean, while in the II group they appeared after four months. Total viability of callus in the studied genotypes is correlated with the ability of callus formation on two types of explants (buds and needles). It is concluded that callusogenesis characteristics may be used to differentiate the genotypes of P. sibirica.