Application of Ellipsometry for Studying Bio-Organic Media
M. I. Voevoda1, S. E. Pel'tek1, M. V. Kruchinina2, S. A. Kurilovich2, V. N. Kruchinin3, S. V. Rykhlitskii3, K. P. Mogil'nikov3
1 Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
2 Institute of Internal Medicine, Siberian Branch, Russian Academy of Medical Sciences
3 Rzhanov Institute of Semiconductor Physics, Siberian Branch, Russian Academy of Sciences
voevoda@iimed.ru, peltek@bionet.nsc.ru, kruchmargo@yandex.ru, kurilovich@yandex.ru, vladd50@mail.ru, rhl@isp.nsc.ru, mogkonst@thermo.isp.nsc.ru
Keywords: ellipsometry, blood serum, pathology of internals, surface plasmon resonance
Pages: 114-121
Abstract
Thin films obtained by centrifugation of blood serum of healthy people and patients with diffuse pathology of liver with different degrees of fibrosis are studied by means of spectroscopic ellipsometry. The physical properties of the films are found to depend on the bio-organic composition of blood serum, which, in turn, is determined by pathological processes proceeding at different degrees of liver fibrosis. Correlations between the bio-chemical parameters of blood serum and ellipsometric data are obtained. Pilot ellipsometric experiments on studying blood serum with observations of the plasmon resonance of patients with intestinal tract tumors are performed. A persistent specific interaction between antigens of patient's blood serum and SD24 monoclonal antibodies is found, which alters the position of the surface plasmon resonance. It is demonstrated that ellipsometry is a high-sensitivity nondestructive inexpensive express method of screening, i.e., preliminary diagnostics of disease stages for patients with pathologies of internals.
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