Ellipsometric Monitoring in Label-Free Microarray Biotechnologies
V. V. Vlasov1, A. N. Sinyakov1, D. V. Pyshnyi1, S. V. Ryhlitskii2, V. N. Kruchinin2, E. V. Spesivtsev2, I. A. Pyshnaya1, E. V. Kostina1, E. D. Dmitrienko1, V. P. Bessmel'tsev3
1 Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences
2 Rzhanov Institute of Semiconductor Physics, Siberian Branch, Russian Academy of Sciences
3 Institute of Automation and Electrometry, Siberian Branch, Russian Academy of Sciences
sinyakov@niboch.nsc.ru, pyshnyi@niboch.nsc.ru, rhl@isp.nsc.ru, kruch@isp.nsc.ru, evs@isp.nsc.ru, bessmelt@iae.nsk.su
Keywords: ellipsometry, biochips, label-free diagnostics, oligonucleotides, proteins
Pages: 67-77
Abstract
The surface of biochips prepared on the basis of silicon wafers for studies of reactions of pre-immobilized oligonucleotides and protein molecules was analyzed by high-resolution scanning ellipsometry. The hybridization interactions of nucleic acid (duplex formation in the reaction of an amplicon of the influenza A virus matrix protein gene with probe molecules), sorption of protein molecules, and protein-protein interactions on the surface of the biochip were detected. It was shown that pre-sorption of gold nanoparticles led to greater efficiency of immobilization of protein molecules on the biochip. The experimental data suggest that that ellipsometry is a highly sensitive, nondestructive and inexpensive label-free method for the detection of the biochip surface, which is suitable for quantitative analysis of reactions of biomolecules.
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